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HPLC Application ÀÚ·á½Ç

St. John's Wort (Hypericum perforatum)
C18, C18(2)

Luna 5u C18(2) 100A 150 x 4.6mm ID

itraconazole ºÐ¼®Ä÷³ ¹× SPE method
Phenyl-hexyl

Based on the structure, the appropriate SPE is starta-X and HPLC column is Luna Phenyl-hexyl 
For strata-X, use the generic method. This compound is not really soluble in water or dilute acids, so dilute plasma with neutral or alkaline buffer before loading onto SPE. Wash with neutral buffer, dry under vacuum, then elute with methanol.
For HPLC on Luna Phenyl-hexyl, I would recommend a neutral pH buffer and methanol. Compound is fairly hydrophobic, so I would start with 50/50 buffer:methanol and adjust as needed.

Phthalate Esters on EnviroSep-PP (EPA method 606)
ENVIROSEP
EnviroSep-PP 125 x 3.2mm
Benzidines on EnviroSep-PP (EPA method 605)
ENVIROSEP
EnviorSep-PP 125 x 3.2mm
Carbamate / Urea Pesticides on EnviroSep-PP (EPA method 632)
ENVIROSEP
EnviroSep-PP 125 x 4.6 mm
PAH with Surrogate Standards on EnviroSep-PP (Flu), EPA method 610
ENVIROSEP
EnviroSep-PP 125 x 4.6mm
PAH with Surrogate Standards on EnviroSep-PP (UV), EPA method 610
ENVIROSEP
EnviroSep-PP 125 x 4.6mm
Alanine ( L-Alanine, D-Alanine )
CHIREX

Chirex 3126 150 x 4.6mm

 

 

Peptides Comparison -Jupiter Proteo
Jupiter Proteo
Jupiter 4¥ì Proteo 250 x 4.6 mm
40 Pesticides out of multi-target screening method for 300 compounds
Fusion-RP

Synergi 4¥ì Fusion-RP 50 x 2.0

Detector : Mass Spectrometer(MS)

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