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Life time

 Axia ÷ Long column life time ʽÿ
 

 

 

Conditions

(same for both columns except where noted)

Column: Luna 5µm C18(2) Axia Packed
Waters XTerra 5µm Prep MS C18 OBD
Dimensions: 50 x 21.2mm (Luna)
50 x 19mm (XTerra)
Mobile Phase: A: 0.5% TFA in Water
B: 0.5% TFA in Acetonitrile
Gradient: Linear 95:5 (A/B) to 5:95 (A/B) over 7 min, hold 3 min
Injection Volume 500 µL
Flow Rates: 30 mL/min (Luna)
24 mL/min (XTerra)
Detection: UV @ 254nm
Sample: 1. Triprolicine 1.6 mg
2. Methacycline 16 mg
3. Amitriptyline 5.25 mg

 
 

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